Journal: Stem Cell Research & Therapy

Article Title: Oxidant therapy improves adipogenic differentiation of adipose-derived stem cells in human wound healing

doi: 10.1186/s13287-021-02336-3

Figure Lengend Snippet: Characterization of the macrophage activation model. a THP1 cell treatment protocol for in vitro polarization of macrophages. b Morphology (phase contrast, scale bar 100μm) of THP1-derived macrophages on day of CM harvest. c Heatmap of log2 cytokine protein levels in differentially activated macrophages. The dendrogram represents the result from a complete linkage hierarchical clustering of mean-centered expression levels based on Euclidian similarity. d mRNA-expression levels of macrophage-selective markers (M (IFNG/LPS) : CD80, IL1B, and M (IL4/IL13) : CD200R, TGM2). Data were normalized to the 18S rRNA reference gene and shown as mean –ΔCt. Asterisks indicate p values < 0.05 (*), p <0.01 (**), and p <0.001 (***). Statistical significance ( n =5) was determined by using one-way ANOVA and Dunnett’s test for multiple comparison

Article Snippet: Macrophage-CM was loaded onto RayBio® Human Cytokine G5 Antibody Microarray Glass Chip (RayBiotech, Norcross, GA) which facilitates the detection of 80 targets.

Techniques: Activation Assay, In Vitro, Derivative Assay, Expressing

Journal: Mediators of Inflammation

Article Title: LR12 Promotes Liver Repair by Improving the Resolution of Inflammation and Liver Regeneration in Mice with Thioacetamide- (TAA-) Induced Acute Liver Failure

doi: 10.1155/2021/2327721

Figure Lengend Snippet: LR12 promoted hepatocyte regeneration via CCL20 secreted by macrophages. (a) The cytokine protein microarray of the supernatant from macrophages. (b) ELISA of CCL20 in the supernatant from macrophages. (c) The mRNA level of CCL20 in macrophages. (d) CLSM showing F4/80 and CCL20 staining in the liver tissues. (e) Western blot analysis of PCNA in LO2 cells stimulated with CCL20. Data were presented as the mean ± standard deviation (SD). ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 versus control group.

Article Snippet: The RayBio Human Cytokine Antibody Array Kit (QAH-TH17-1-1 microarray; RayBiotech, Norcross, GA, USA) was utilized to identify the cytokines based on instructions provided by the manufacturer.

Techniques: Microarray, Enzyme-linked Immunosorbent Assay, Staining, Western Blot, Standard Deviation

Journal: Science Advances

Article Title: Fructose-1,6-bisphosphate prevents pregnancy loss by inducing decidual COX-2 + macrophage differentiation

doi: 10.1126/sciadv.abj2488

Figure Lengend Snippet: ( A ) Heatmap of the differential proteins (differential fold > 3) of supernatants between FBP (0.5 mM)–ESC ( n = 3) and Ctrl-ESC ( n = 3) by the proteomic microarray. ( B ) The IL-27 levels in ESCs ( n = 9) treated with FBP were detected by FCM. ( C ) The IL-27 levels in supernatants of control ESCs ( FBP1 -NC, n = 9) or FBP1 -overexpressing ESCs ( FBP1 over , n = 9) treated with DMSO (1‰) or progesterone (P; 10 −8 μM) were detected by ELISA. ( D ) Expression of IL-27 between control endometrium ( n = 6) and decidua tissues ( n = 6) was detected by immunohistochemistry. ( E to G ) Photograph (red arrow: absorption point) of uterus, absorption rates, or CRL of embryos from WT pregnant mice (mated with male Il27ra −/− mice, n = 8) or Il27ra −/− pregnant mice (mated with male WT mice, n = 8). ( H ) Transcription levels of decidualization-related genes in uterus from (E) were analyzed by RT-PCR. ( I ) Depth of CK7 + trophoblast infiltration into uterus from (E) was observed by hematoxylin and eosin staining or immunofluorescence assays. DAPI, 4′,6-diamidino-2-phenylindole. Data were presented as means ± SEM and analyzed by t test or one-way ANOVA test. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Article Snippet: After stimulation with FBP (0.5 mM) or without, the supernatants from Ctrl-ESC (secretory phase, n = 3) and FBP-ESC (secretory phase, n = 3) were collected and analyzed by the proteomic microarray RayBio L-Series Human Antibody Array 1000 (RayBiotech Inc., GA, USA) as described previously ( ).

Techniques: Microarray, Enzyme-linked Immunosorbent Assay, Expressing, Immunohistochemistry, Reverse Transcription Polymerase Chain Reaction, Staining, Immunofluorescence

Journal: Science Advances

Article Title: Fructose-1,6-bisphosphate prevents pregnancy loss by inducing decidual COX-2 + macrophage differentiation

doi: 10.1126/sciadv.abj2488

Figure Lengend Snippet: ( A ) Expression of COX-2 in uMφs of WT (mated with male Il27ra −/− mice, n = 8) or Il27ra −/− pregnant mice (mated with male WT mice, n = 8) was detected by FCM. ( B ) Expression of COX-2 in uMφs of saline (1%, n = 8) or 2-DG (50 mg/kg per day, n = 8)–treated pregnant mice was detected by FCM. ( C ) Expression of differentiation molecules in uMφs of WT ( n = 8) or Ptgs2 −/− pregnant mice ( n = 8) was detected by FCM. ( D to F ) Photograph (red arrow: absorption point) of uterus, the CRL of embryos, or absorption rates from WT ( n = 8) or Ptgs2 −/− pregnant mice ( n = 8). ( G ) Differential proteins of supernatants between IL-27RA + dMφs and IL-27RA − dMφs were evaluated by the proteomic microarray. ( H to J ) Transcription levels of decidualization-related genes in uterus, depth of CK7 + trophoblast infiltration into uterus, and embryo absorption rates of Il27ra −/− pregnant mice (mated with male WT mice, n = 8) adopted with WT or Ptgs2 −/− macrophages. Data were presented as means ± SEM or median and quartile and analyzed by t test, Mann-Whitney U test, or one-way ANOVA test. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: After stimulation with FBP (0.5 mM) or without, the supernatants from Ctrl-ESC (secretory phase, n = 3) and FBP-ESC (secretory phase, n = 3) were collected and analyzed by the proteomic microarray RayBio L-Series Human Antibody Array 1000 (RayBiotech Inc., GA, USA) as described previously ( ).

Techniques: Expressing, Microarray, MANN-WHITNEY

Journal: Journal of Clinical Laboratory Analysis

Article Title: Increased CCL24 and CXCL7 levels in the cerebrospinal fluid of patients with neurosyphilis

doi: 10.1002/jcla.23366

Figure Lengend Snippet: Chemokine array performed on four patients with neurosyphilis and four with non‐neurosyphilis. A, Full chemokine array paradigm. B, Digital images taken with a chemiluminescence imaging system. C, Signal intensities recognized by ImageJ with Protein Array Analyzer plugin. D, Relative signal intensity of 38 chemokines in four patients with neurosyphilis and four with non‐neurosyphilis. CXCL13 is indicated by the red rectangle, CCL24 by the yellow rectangle, CXCL8 by the green rectangle, CXCL10 by the blue rectangle, and CXCL7 by the purple rectangle

Article Snippet: The CSF samples were evaluated using a protein array (RayBio ® Human Chemokine Antibody Array C1 Kit; RayBiotech), according to the manufacturer's instructions.

Techniques: Imaging, Protein Array

Journal: Journal of Clinical Laboratory Analysis

Article Title: Increased CCL24 and CXCL7 levels in the cerebrospinal fluid of patients with neurosyphilis

doi: 10.1002/jcla.23366

Figure Lengend Snippet: Receiver operating characteristic curve analyses of cerebrospinal fluid (CSF) and serum chemokine biomarkers in discrimination of neurosyphilis. A and B, Area under the curve (AUC) of CSF (A) and serum (B) levels of CCL24. C and D, AUC of CSF (C) and serum (D) levels of CXCL7

Article Snippet: The CSF samples were evaluated using a protein array (RayBio ® Human Chemokine Antibody Array C1 Kit; RayBiotech), according to the manufacturer's instructions.

Techniques:

Journal: Journal of Visualized Experiments : JoVE

Article Title: Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

doi: 10.3791/3791

Figure Lengend Snippet:

Article Snippet: However, customized antibody microarray manufacture can be readily done from a service provider such as Serome Biosciences Inc. For a robust result, we recommend a non-contact microarrayer, such as the Scienion sciFLEXARRAYER ultra low volume non-contact microarrayer which was used in our antibody microarray manufacture.

Techniques: Microarray, Staining, Incubation, Protease Inhibitor